POLYCYSTIN-L1; RBX-100UL

Code: AB9084 D2-231

Analysis Note

ControlPOSITIVE CONTROL: adult mouse retina

NEGATIVE CONTROL: adult mouse lung

Application

Research Sub CategoryRenal P...


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€529.00 EACH
€650.67 inc. VAT

Analysis Note

ControlPOSITIVE CONTROL: adult mouse retina

NEGATIVE CONTROL: adult mouse lung

Application

Research Sub CategoryRenal Physiology

Western blot: 1:1,000 using ECL. The antibody reacts with the 90 kDa Polycystin1-L1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

This Anti-Polycystin-L Antibody is validated for use in WB, IC, IH for the detection of Polycystin-L.

Research CategoryMetabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide from the N-terminal of human Polycystin-L1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

Polycystin-L

antibody formserum
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationmouse ... Pkd2(18764)
manufacturer/tradenameChemicon®
NCBI accession no.NM_138295.2
Quality Level100
shipped indry ice
species reactivitymouse
technique(s)immunocytochemistry: suitable, western blot: suitable, immunohistochemistry: suitable
UniProt accession no.Q9P0L9
This product has met the following criteria to qualify for the following awards:



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